Autoradiograms out-of steroid metabolites of vitellogenic ovarian hair follicles of bambooleaf wrasse incubated that have [7- 3 H]pregnenolone

Autoradiograms out-of steroid metabolites of vitellogenic ovarian hair follicles of bambooleaf wrasse incubated that have [7- 3 H]pregnenolone

Seven metabolites were split of the thin level chromatography and you can set-up that have benzene:acetone (4:1). 1, E1; 2, AD; 3, P5; 4, DHEA 17-P; 5, E2; 6, 1eight-P5; eight, 17,20?-P 17,21-P; and you may 8, 17,20?-P5. Pick Desk 1 having steroid abbreviations.

Table step three

To determine the steroidogenic pathway out-of E2 synthesis, four other radiolabeled steroids were used just like the precursors to possess into the vitro incubation out-of follicles. Ones four precursors, E2 are synthesized of [ 14 C]DHEA, [ fourteen C]Offer, and [ step three H]E1. When hair follicles were incubated which have [ step 3 H]17-P, E2 wasn’t perceived because metabolite, and you may a small amount of Advertisement is introduced. In most incubations, T production could not become detected, and [ 14 C]T was not entirely changed into E2.

Dimensions from solution steroid accounts

Gel levels of E2 and you will E1 shown equivalent profiles in the a great day of spawning season (Fig. (mehr …)